Although the conversion of cellulose to polyols is currently well-developed, the production of the considerably valuable ethylene glycol (EG) is still challenging. Reactions have long relied on the design of suitable catalysts to obtain a high selectivity and yield of EG. Herein, using well-shaped rectangular tungsten trioxide nanosheets as the substrate, we investigated the catalytic performances of various metal supported catalysts for the convertion of cellulose to EG. Results show that Ru/WO3 is more favorable for EG production, with the highest EG yield of 76.3% over the 1% Ru/WO3 nanosheet catalyst. Our characterizations and activity tests suggest that the embedding of Ru nanoparticles onto the WO3 nanosheets produces more W5+ active sites under the same reduction conditions (NaBH4 or H-2), which act as Lewis base sites to promote the glucose retro-aldol condensation reaction. Moreover, the Ru/WO3 catalyst holds a portion of Ru in the form of amorphous RuOx delta+ phases, which could further increase the H+ released into an aqueous solution for cellulose hydrogenolysis. A possible catalytic mechanism for this hydrogenolysis process is accordingly proposed.