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  • Enzyme Mimic Based on a Self-Assembled Chitosan/DNA Hybrid Exhibits Superior Activity and Tolerance
  • 来源:王焱教授个人网站 2020-01-26

  •         Nature has evolved enzymes with exquisite active sites that

    catalyze biotransformations with high efficiency. However, the

    exploitation of natural enzymes is often hampered by poor stability, and

    natural enzyme production and purification are costly. Supramolecular

    self‐assembly allows the construction of biomimetic active sites,

    although it is challenging to produce such artificial enzymes with

    catalytic activity and stability that rival those of natural enzymes. We

    report herein a strategy to produce a horseradish peroxidase (HRP)

    mimic based on the assembly of chitosan with a G‐quadruplex DNA

    (G‐DNA)/hemin complex. A network‐like morphology of the assembled

    nanomaterial was observed together with a remarkable enhancement of

    peroxidase activity induced by the chitosan and G‐DNA components. The

    turnover frequency and catalytic efficiency of the enzyme‐mimicking

    material reached or even surpassed those of HRP. Moreover, the catalytic

    complex exhibited higher tolerance than HRP to harsh environments, such

    as extremely low pH or high temperatures. In accord with the

    experimental and simulated results, it is concluded that the spatial

    distribution of the G‐DNA and chitosan components and the exposure of

    the catalytic center may facilitate the coordination of substrates by

    the hemin iron, leading to the superior activity of the material. Our

    work provides a simple and affordable avenue to produce highly active

    and robust enzyme‐mimicking catalytic nanomaterials.

         

  • [来源:中国聚合物网]
  • 了解更多请进入: 王焱教授个人网站

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