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胍基阳离子聚合物与DNA相互作用
关键字：阳离子 胍基 聚合物-DNA复合物
在基因治疗的实施中，有四个重要的环节[1，2]：一是寻找有效治疗基因，二是可以携带基因进入靶细胞进行表达的安全有效转基因载体，三是选择合适有效的转基因途径，四是使治疗基因在靶细胞中特异性表达并发挥治疗作用。其中缺乏有效安全的转基因载体是制约基因治疗实施的一个主要瓶颈。非病毒载体与病毒载体相比，其优点主要体现在非病毒载体很低免疫原性，不具备插入突变的能力。但非病毒载体的最大缺点是其转基因效率较低。理想的...
http://www.polymer.cn/research/dis_info4077 |
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PEG-PDMA包覆DNA的研究
关键字：聚乙二醇－聚(甲基丙烯酸二甲胺乙酯)，DNA包覆，酶切
近几年来，基因治疗成为当前研究的热点，由于病毒基因载体的局限性，非病毒基因载体受到了广泛的关注，设计并合成运载DNA的非病毒载体成为众多科研工作者竞相研究的课题。由于DNA在生理条件下带负电，阳离子型聚合物可以自发地与DNA结合并将其包裹[1]，阻止酶降解DNA，因此，阳离子型聚合物，尤其是阳离子型嵌段共聚物成为重点研究对象[2] 。聚乙二醇－聚(甲基丙烯酸二甲胺乙酯)（PEG-PDMA）是一种pH敏感型聚合物，在pH7.2时...
http://www.polymer.cn/research/dis_info4019 |
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Neutron Scattering Studies of the Structure and Dynamics of Water Around DNA/proteins, Amino Acids and Biopolymers
关键字：Neutron,Scattering,Studies,Structure,Dynamics,Water,Around,DNA,proteins,Amino,Acids,Biopolymers,
The interaction of water with DNA, proteins and biopolymers with respect to their structure and function is emerging field of neutron community.
http://www.polymer.cn/research/dis_info2257 |
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Complexes Formed by Plasmid DNA Interacting with Poly(ethylene glycol)-b-poly(L-lysine) in Aqueous and Non-aqueous Solutions
关键字：Complexes,Formed,Plasmid,DNA,Interacting,Poly,ethylene,glycol,poly,lysine,Aqueous,Non,aqueous,Solutions,
To successfully deliver the transgenes to cell nucleus with the aid of non-viral vectors, the DNA complexes should possess certain structure or properties to overcome the barriers along the delivery pathway.
http://www.polymer.cn/research/dis_info2221 |
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Novel QDs/AuNPs Conjugates Based on Fluorescence Resonance Energy Transfer in DNA Detection
关键字：Novel,QDs,AuNPs,Conjugates,Based,Fluorescence,Resonance,Energy,Transfer,DNA,Detection,
In recent years, inorganic nanoparticles (NPs) have become the subject of intensive research for detection and labeling of biomolecules due to their size-dependent physical and chemical characteristics.[1] As a result, a variety of opt...
http://www.polymer.cn/research/dis_info2051 |
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Novel QDs/AuNPs Conjugates Based on Fluorescence Resonance Energy Transfer in DNA Detection
关键字：Novel,QDs,AuNPs,Conjugates,Based,Fluorescence,Resonance,Energy,Transfer,DNA,Detection,
In recent years, inorganic nanoparticles (NPs) have become the subject of intensive research for detection and labeling of biomolecules due to their size-dependent physical and chemical characteristics.
http://www.polymer.cn/research/dis_info1944 |
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Synthesis and performance study of polymer/metal composite matrices for DNA sequencing by capillary electrophoresis
关键字：Synthesis,performance,study,polymer,metal,composite,matrices,DNA,sequencing,capillary,electrophoresis,
Fast, highly efficient, and sensitive capillary electrophoresis (CE), including capillary array electrophoresis (CAE) and microchip electrophoresis (MCE), is one of the most important techniques for DNA analysis. During the separation and sequen...
http://www.polymer.cn/research/dis_info1623 |
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Modeling single chain elasticity of single-stranded DNA: A comparison of three models
关键字：single chain elasticity
The
dominant models of polymers, i.e. the freely jointed chain (FJC) model, the wormlike
chain (WLC) model and the freely rotating chain (FRC) model are modified by
integrating the inherent single-molecule elasticity obtained from quantum
mechanics (QM) ab-initio calculations. The QM modified models have been
utilized to generate fitting curves for single-stranded DNA obtained in organic
solvent. ...
http://www.polymer.cn/research/sy_info19131 |
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Double Stranded DNA Dissociates into Single Strands when Dragged into a Poor Solvent
关键字：DNA, single molecule
DNA displays a richness of biologically relevant
supramolecular structures, which depend on both sequence and ambient. The effect of dragging
dsDNA from water into poor solvent on the double-stranded structure is still
unclear because of condensation. Here
we employ single molecule techniques based on atomic force microscopy and molecular dynamics (MD) simulations to investigate the change in...
http://www.polymer.cn/research/sy_info19127 |
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Self-assembly of water-soluble conjugated polymers on solid surface for cellular imaging and DNA assay
Conjugated polymers (CPs) have proven to be attractive materials for various optoelectronic applications including light-emitting diodes, field-effect transistors, and photovoltaic devices due to their excellent photo- and electroluminescent properties.
http://www.polymer.cn/research/dis_info16729 |
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