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高分子溶液分离DNA：链刚性和临界交叠浓度的影响
关键字：高分子,溶液,分离,DNA,刚性,临界,交叠,浓度,影响,
水溶性高分子在浓度高于C*时，会通过分子链间的缠结作用形成具有特定网孔大小与分布的网络结构。一般认为，正是由于这种网络结构通过筛分（sieving）作用实现了对DNA 的分离。但也有文献报道，羟已基纤维素在浓度远低于临界交叠浓度 (C*) 时，也能够对DNA 进行有效的分离，其主要原因是由于刚性高分子在DNA 的迁移过程中与DNA 链发生瞬间的缠结，而且这种缠结的频率和时间DNA 片断的大小有关，...
http://www.polymer.cn/research/dis_info3826 |
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A Strategy for Dramatically Enhancing the Selectivity of Molecules Showing Aggregation-Induced Emission towards Biomacromolecules with the Aid of Graphene Oxide
关键字：biosensors, DNA, energy transfer, graphene, proteins
By intelligently utilizing the different interacting strengths between different moieties according to the displacement method, general biosensors with aggregation-induced emission (AIE) characteristics for biomacromolecules without selectivity were converted
to excellent, highly selective probes for one specific biomacromolecule with the aid of graphene oxide (GO) in an aqueous medium. Importan...
http://www.polymer.cn/research/sy_info17354 |
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DNA、水环境和化学进化一单分子力谱研究与思考
DNA 是生命的关键分子，其力学行为涉及到生命活动的方方面面。我们利用原子力显微镜研究了DNA的单分子力谱。研究结果表明，在有机溶剂中单链DNA 的拉伸力学行为与量子力学从头算给出的结果一致，此时其力学行为对应于纯弹性。
http://www.polymer.cn/research/dis_info13180 |
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A Strategy for Dramatically Enhancing the Selectivity of Molecules Showing Aggregation-Induced Emission towards Biomacromolecules with the Aid of Graphene Oxide
关键字：biosensors, DNA, energy transfer, graphene, proteins
By intelligently utilizing the different interacting strengths between different moieties according to the displacement method, general biosensors with aggregation-induced emission (AIE) characteristics for biomacromolecules without selectivity were converted
to excellent, highly selective probes for one specific biomacromolecule with the aid of graphene oxide (GO) in an aqueous medium. Importan...
http://www.polymer.cn/research/sy_info17354 |
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DNA、水环境和化学进化一单分子力谱研究与思考
DNA 是生命的关键分子，其力学行为涉及到生命活动的方方面面。我们利用原子力显微镜研究了DNA的单分子力谱。研究结果表明，在有机溶剂中单链DNA 的拉伸力学行为与量子力学从头算给出的结果一致，此时其力学行为对应于纯弹性。
http://www.polymer.cn/research/dis_info13180 |
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Reversibly Shielded DNA Polyplexes Based on Bioreducible PDMAEMA-SS-PEG-SS-PDMAEMA Triblock Copolymers Mediate Markedly Enhanced Nonviral Gene Transfection
Reversibly shielded DNA polyplexes based on bioreducible poly(dimethylaminoethyl methacrylate)-SS-poly(ethylene glycol)-SS-poly(dimethylaminoethyl methacrylate) (PDMAEMA-SS-PEG-SS-PDMAEMA) triblock copolymers were designed, prepared and investigated for in vitro gene transfection. Two PDMAEMA-SS-PEG-SS-PDMAEMA copolymers with controlled compositions, 6.6–6–6.6 and 13–6–13 k...
http://www.polymer.cnt//ss/zhongzhiyuan/publicationsshow_4791.html |
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Cationic methacrylate copolymers containing primary and tertiary amino side groups: Controlled synthesis via RAFT polymerization, DNA condensation, and in vitro gene transfection
A versatile family of cationic methacrylate copolymers containing varying amounts of primary and tertiary amino side groups were synthesized and investigated for in vitro gene transfection. Two different types of methacrylate copolymers, poly(2-(dimethylamino)ethyl methacrylate)/aminoethyl methacrylate [P(DMAEMA/AEMA)] and poly(2-(dimethylamino)ethyl methacrylate)/aminohexyl methacrylate [P(DMAEMA...
http://www.polymer.cnt//ss/zhongzhiyuan/publicationsshow_3001.html |
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Biomaterials 2009: Transfection and intracellular trafficking characteristics for poly(amidoamine)s with pendant primary amine in the delivery of plasmid DNA to bone marrow stromal cells
Poly(amidoamine)s with pendant primary amine (polymer 1a-1c) were evaluated as in vitro non-viral gene delivery vectors for bone marrow stromal cells (BMSCs). The cytotoxicity of these poly(amidoamine)s, measured by MTT assay, increased with increasing length of side chain, however, they were less toxic than branched polyethylenimine (PEI) 25 kDa. Using pGL-3 and pEGFP-C1 as luciferase gene and gr...
http://www.polymer.cnt//ss/huangshiwen/publicationsshow_2016.html |
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Random and block copolymers of bioreducible poly(amido amine)s with high- and low-basicity amino groups: Study of DNA condensation and buffer capacity on gene transfection
Poly(amido amine) (SS-PAA) random and block copolymers having bioreducible disulfide bonds in the main chain and amino groups with distinctly different basicity in the side chain were designed and synthesized by Michael addition polymerization between N, N'-cystaminebisacrylamide (CBA) and two amine monomers, i.e., histamine (HIS) and 3-(dimethylamino)-1-propylamine (DMPA). Copolymers containing v...
http://www.polymer.cnt//ss/zhongzhiyuan/publicationsshow_1012.html |
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双链DNA 在外力诱导下构象转变本质的单分子力谱研究
关键字：单分子力谱，DNA-蛋白质相互作用，DNA力学指纹谱
基于原子力显微镜技术的单分子力谱可以实现对单个大分子进行力学操纵和加工，从而对分子结构与构象变化，分子间的相互作用以及反应历程实现单分子水平的实时-原位观测。目前单分子力学谱已经成为生物物理，高分子科学，表面学和细胞分子生物学等领域不可或缺的一种重要的研究手段。
http://www.polymer.cn/research/dis_info10716 |
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