Calcium-dependent Hydrolysis of Supported Planar Lipids Were Triggered by honey bee venom Phospholipase A2 with Right Orientation at Interface
作者：Siqi Kai, Xu Li, Bolin Li, Xiaofeng Han, Xiaolin Lu*
关键字：PLA2 orientation, Ca2+, Lipids, SFG, LSCM
论文来源：期刊
具体来源：Phys. Chem. Chem. Phys. 2018, 20, 63-67
发表时间：2018年
我们结合三种光学技术解析了界面上磷脂酶水解磷脂分子的过程，看到了水解的动力学过程以及水解产物在界面上的分布。其中，证实了酶的取向对于界面水解的功能至关重要。这对于我们后续的研究具有直接的指导意义，我们的后续研究已经开展！
This paper was published as a back cover one in PCCP.
We combined sum frequency generation (SFG) vibrational spectroscopy, laser scanning confocal microscopy (LSCM) and attenuated total reflection infrared spectroscopy (ATR-IR) to study the hydrolysis of phospholipids catalyzed by a Ca2+-dependent enzyme, honey bee venom phospholipase A2 (bvPLA2). The experiments demonstrated that Ca2+ ions mediated between the lipids and bvPLA2, induced reorientation of bvPLA2, and activated hydrolysis. One of hydrolysis products, fatty acids, was desorbed, and the other one, lysophospholipids, self-organized at the interface. This study provides a refreshing and vivid picture at the molecular and micron levels for people to understand the Ca2+-dependent interfacial lipid hydrolysis catalyzed by bvPLA2.
http://pubs.rsc.org/en/content/articlehtml/2017/cp/c7cp06344j