相关链接
联系方式
  • 通信地址:苏州市十梓街1号 苏州大学 软凝聚态物理及交叉研究实验室
  • 邮编:215006
  • 电话:0512-65884406
  • 传真:
  • Email:gaojian.chen@gmail.com
当前位置:> 首页 > 最新动态 > 正文
祝贺李志运在Macromol. Rapid Commun.上发表研究论文

Li, Z., Zhang, P., Lu, W., Peng, L., Zhao, Y., & Chen, G. (2016). Ratiometric Fluorescent pH Probes Based on Glycopolymers. Macromolecular Rapid Communications, 37(18), 1513–1519. http://doi.org/10.1002/marc.201600242


Effectively detecting pH changes plays a critical role in exploring cellular functions and determining physiological and pathological processes. A novel ratiometric pH probe based on a glycopolymer, armored with properties of serum-stability, tumor-targeting, and pH monitoring, is designed. Random copolymers of 2-(methacrylamido) glucopyranose and fluorescein O-methacrylate are first synthesized by reversible addition fragmentation chain transfer polymerization. Acryloxyethyl thiocarbamoyl rhodamine B is then attached to the polymer chain to prepare ratiometric fluorescent pH probes via a thiol-ene reaction. The synthesized polymeric probes are characterized by NMR, gel permeation chromatography, UV–vis spectroscopy, and transmission electron microscopy, and the fluorescence responses are examined in phosphate buffer at different pHs. The cytotoxicity and confocal imaging experiments of the probes are detected using HeLa cells, demonstrating a low toxicity and superior biocompatibility for detecting pH changes in bioapplications.