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【[Journal of Dairy Science]】Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk
作者:Jiaojiao Han,Lei Zhang, Liming Hu, Keyu Xing, Xuefei Lu, Youju Huang, * Jiawei Zhang, Weihua Lai, Tao,Chen
关键字:Nanozyme-based lateral flow assay
论文来源:期刊
具体来源:Journal of Dairy Science
发表时间:2018年

The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip. Due to a synergy between two metals and high-index facets of concave structure, bimetallic concave nanoparticles shows significantly enhanced cataltic performance. In this study, a kind of nanozymes, which was bimetal Pd-Pt concave nanoparticles, was employed as the probe to develop a sensitive LFA based on sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity was improved by applying the 3,3’,5,5’-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated with the presence of analytes. The nanozyme showed high catalytic performance toward TMB, and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA were 0.87×102 cfu/mL in PBS and 9.0×102 cfu·mL-1 in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed Pd-Pt  nanoparticles labled LFA shows remarkable potential for detecting various pathogens in real samples.