writer：Chang Zhang, Jing Jin, Jie Zhao, Wei Jiang, Jinghua Yin
keywords：血液相容性, 表面改性, 聚丙烯无纺布膜, 牛血清白蛋白, 聚丙烯酸, 紫外辐照
source：期刊
specific source：Colloids and Surfaces B: Biointerfaces, 2013, 102, 45-52
Issue time：2013年

以聚丙烯酸（PAA）为间隔层，将牛血清白蛋白（BSA）成功固定到聚丙烯无纺布（PPNWF）膜上。首先将O2等离子体处理与紫外辐照技术相结合，在膜上接枝PAA；随后通过BSA氨基与PAA羧基的偶联反应将BSA固定到PAA接枝表面。ATR-FTIR、XPS和水接触角测试证实了PAA与BSA的成功固定，水接触角结果表明改性后膜的亲水性提高。BSA固定后，改性膜表面的蛋白质吸附量和血小板黏附数显著降低，血液相容性较未改性及PAA接枝PPNWF明显改善；全血凝固时间测定表明改性膜的抗凝血性能也显著增强。

Bovine serum albumin (BSA) was successfully immobilized onto polypropylene non-woven fabric (PPNWF) membranes using poly(acrylic acid) (PAA) as a spacer. O2 plasma treatment and UV-irradiated technique were combined to graft PAA onto the membranes, and BSA was then immobilized onto the PAA grafted surface through the coupling of amino groups of BSA to the carboxyl groups of PAA. The immobilization was confirmed by ATR-FTIR, XPS and water contact angle measurement. After BSA immobilization, the amount of protein adsorption and the number of platelet adhesion on the modified membrane significantly decreased, and the whole blood clotting time measurement showed that the anticoagulant property was significantly enhanced.

论文链接：https://doi.org/10.1016/j.colsurfb.2012.08.007