writer：Yanqiu Du, Jing Jin, Wei Jiang
keywords：聚乙二醇, 回填, 靶标识别, QCM-D, DPI, 防污
source：期刊
specific source：Journal of Materials Chemistry B, 2018, 6(39), 6217-6224
Issue time：2018年

摘要：聚乙二醇（PEG）在生物医学应用中具有蛋白质抵抗和保持蛋白质功能的潜力。本文设计了一种基于PEG的双功能平台，对血浆蛋白具有防污性且对生物分子具有高灵敏度。使用长PEG链（PEG24）安装功能性生物分子，短PEG链（PEG4）作为保护层回填表面并抑制非特异性蛋白质吸附。结合带耗散功能的石英晶体微天平（QCM-D）和双偏振干涉技术（DPI），实时研究了PEG4回填的动态过程以及不同PEG4/PEG24比例下生物分子的识别能力。PEG4回填量影响PEG24的柔性和暴露位点，增加PEG4与PEG24的比例可提高识别能力。因此，当PEG4与PEG24的投料比为9:1时，构建了高效、灵敏的平台，可在纯PBS或复杂生物环境中固定抗体并识别抗原。

Abstract：Polyethylene glycol (PEG) is a promising candidate for protein resistance and preserving protein function in biomedical applications. In this study, a PEG-based bifunctional platform with antifouling for plasma proteins and high sensitivity for biomolecules was designed. Long PEG chains (PEG24) were used to install functional biomolecules, and short PEG chains (PEG4) served as a protective layer to backfill the surface and suppress nonspecific protein adsorption. Quartz crystal microbalance with dissipation (QCM-D) and dual polarization interferometry (DPI) were combined to investigate the dynamic process of PEG4 backfilling and the recognition capacity of biomolecules with different ratios of PEG4 and PEG24 in real time. The amount of PEG4 chain backfilling affected the flexibility of PEG24 and exposed sites. The recognition capacity was improved by increasing the ratios of PEG4 to PEG24. Therefore, when the feeding ratio of PEG4 to PEG24 was 9:1, a highly efficient and sensitive platform was constructed for immobilization of antibodies and recognition of antigens either in pure PBS or in a complex biological environment.

论文链接：https://doi.org/10.1039/c8tb01526k