writer：Jianhua Lv, Jing Jin, Jiayue Chen, Bing Cai, Wei Jiang
keywords：赖氨酸衍生物, 表面改性, 防污, 抗菌, SEBS
source：期刊
specific source：ACS Applied Materials & Interfaces, 2019, 11(28), 25556-25568
Issue time：2019年

摘要：血液相容性和抗菌性能对于血液接触装置和医疗介入材料至关重要。本文将带正电荷的季铵（QAC）和疏水苄基（OBzl）引入亲水赖氨酸甲基丙烯酰胺（LysAAm），分别获得两种单体LysAAm-QAC和LysAAm-OBzl，并通过质子核磁共振、傅里叶变换红外光谱和飞行时间二次离子质谱对其结构进行表征。将LysAAm-QAC和LysAAm-OBzl以不同投料比共接枝到硅片上构建防污和抗菌性能。纤维蛋白原吸附和血小板黏附结果表明，投料比为3:7的改性样品具有优异防污性能；2小时和24小时抗菌测试均表明投料比3:7的改性样品具有抗菌能力。防污性能源于LysAAm-QAC和LysAAm-OBzl的高表面覆盖率（91.49%）以及LysAAm-QAC和LysAAm-OBzl上亲水主体结构LysAAm（水接触角43.6°）。随着LysAAm-OBzl比例增加（43.6%-58.5%），抗菌性能提高，因为增加的疏水OBzl增强了插入细菌膜的能力并提高杀菌效率。应用中将投料比3:7的LysAAm-QAC和LysAAm-OBzl接枝到聚苯乙烯-b-(乙烯-co-丁烯)-b-聚苯乙烯表面，制备了兼具防污和抗菌性能的双功能表面，在血液接触装置和医疗介入材料方面具有应用前景。

Abstract：Hemocompatibility and antibacterial property are essential for blood contact devices and medical intervention materials. In this study, positively charged quaternary ammonium (QAC) and hydrophobic benzyl group (OBzl) were introduced onto hydrophilic lysine methacrylamide (LysAAm) to obtain two monomers LysAAm-QAC and LysAAm-OBzl, respectively. The structure characterizations of LysAAm-QAC and LysAAm-OBzl were determined by proton nuclear magnetic resonance, Fourier transform infrared spectroscopy, and time-of-flight secondary ion mass spectrometry. LysAAm-QAC and LysAAm-OBzl were cografted onto a silicon wafer with different feeding ratios to construct antifouling and antibacterial properties. The results of fibrinogen adsorption and platelet adhesion proved that the modified sample with the feeding ratio of 3:7 had superior antifouling property. Furthermore, an antimicrobial test with both 2 and 24 h indicated that the modified sample with the feeding ratio of 3:7 had antibacterial ability. The antifouling property was provided by the high surface coverage of LysAAm-QAC and LysAAm-OBzl (91.49%) and the hydrophilic main structure LysAAm on LysAAm-QAC and LysAAm-OBzl (water contact angle was 43.6°). The antibacterial property was improved with the proportion of LysAAm-OBzl (43.6-58.5%) because the increasing hydrophobic OBzl enhanced the ability to insert into the membrane of bacteria and raise the bactericidal efficiency. In application, LysAAm-QAC and LysAAm-OBzl with the feeding ratio of 3:7 were grafted onto the surface of poly(styrene-b-(ethylene-co-butylene)-b-styrene), and a bifunctional surface with antifouling and antibacterial properties was fabricated, which had promising applications in blood contact devices and medical intervention materials.

论文链接：https://doi.org/10.1021/acsami.9b06281