writer：Yanqiu Du, Jing Jin, Haojun Liang, Wei Jiang
keywords：金纳米粒子, 线性聚合物, 环形聚合物, 生物相容性, 蛋白质抵抗, PEG
source：期刊
specific source：Langmuir, 2019, 35(26), 8316-8324
Issue time：2019年

摘要：具有线性和环形构象的各种聚合物刷在生物材料和纳米技术应用中受到广泛关注。本文合成了基于PEG-SH和SH-PEG-SH链结合金纳米粒子（AuNPs）的线性和环形聚合物刷壳，系统研究了PEG化AuNPs的结构和拓扑，并深入测定了水合尺寸、接枝密度、亲水性、胶体稳定性和生物相容性等基本理化参数。与线性对应物相比，环形聚合物拓扑可显著改变聚合物刷的理化性质。当PEG分子量较低（1和5 kDa）时，环形聚合物壳的水合尺寸和接枝密度小于线性对应物；当PEG分子量为10 kDa时，环形壳比线性壳更厚更致密。有趣的是，AuNPs上的环形PEG在高盐环境中更稳定且亲水性更好，从而赋予优异的生物相容性，包括蛋白质抵抗和细胞活性。这些结果为在AuNPs上设计不同拓扑的聚合物刷、提高杂化AuNPs生物相容性并获取生物医学领域潜在应用提供了一种简单方法。

Abstract：Various polymer brushes with linear and looped conformations have gained considerable attention in the application of biomaterials and nanotechnology. In this work, the linear and looped polymer brush shells based on PEG-SH and SH-PEG-SH chains binding to gold nanoparticles (AuNPs) are synthesized. The structure and topology of the PEGylated AuNPs are systematically investigated. The basic physicochemical parameters of these PEGylated AuNPs such as hydrodynamic size, grafting density, hydrophilicity, colloidal stability, and biocompatibility are determined intensively. The looped polymer topology can remarkably alter physicochemical properties of polymer brushes compared with the linear counterparts. When the molecular weight of PEG is low (1 and 5 kDa), the looped polymer shells have smaller hydrodynamic size and lower grafting density than their linear analogues; when the molecular weight of PEG is high (10 kDa), the looped shells are much thicker and denser than the linear ones. Interestingly, the looped PEGs on AuNPs are more stable in a high-salt environment and have better hydrophilicity, which endow excellent biocompatibility, including protein resistance and cell viability. These results provide a simple approach to design polymer brushes with different topologies on AuNPs, improve the biocompatibility of hybrid AuNPs, and acquire the potential application in the biomedical field.

论文链接：https://doi.org/10.1021/acs.langmuir.9b00045