作者：Jing Jin, Wei Jiang, Jinghua Yin, Xiangling Ji, Paola Stagnaro
关键字：蛋白质吸附, 聚乙二醇, 石英晶体微天平, 血液相容性, 聚乙烯, 牛血清白蛋白, 纤维蛋白原
论文来源：期刊
具体来源：Langmuir, 2013, 29(22), 6624-6633
发表时间：2013年

以接枝两种不同分子量单甲氧基聚乙二醇（mPEG）的聚乙烯为模型，利用带耗散功能的石英晶体微天平（QCM-D）阐明了血浆蛋白的吸附机制。结合血小板黏附、全血凝固时间和溶血率数据，PE-g-mPEG膜的血液相容性显著提高。结果表明，预吸附的牛血清白蛋白（BSA）能有效抑制随后纤维蛋白原（Fib）的吸附；BSA的非特异性吸附由表面覆盖度决定，而与PEG链长无关，致密的PEG刷可释放更多束缚水以抵抗BSA吸附。高浓度BSA可逐渐置换预吸附的Fib，而Fib的吸附与置换受表面亲水性控制。

Monomethoxy poly(ethylene glycol) (mPEG) with two different molecular weights was grafted on polyethylene as a model to elucidate the adsorption mechanisms of plasma protein through quartz crystal microbalance with dissipation (QCM-D). Combined with data from platelet adhesion, whole blood clotting time and hemolysis rate, the blood compatibility of PE-g-mPEG film was significantly improved. The preadsorbed bovine serum albumin (BSA) could effectively inhibit subsequent adsorption of fibrinogen (Fib). Nonspecific protein adsorption of BSA was determined by surface coverage rather than PEG chain length, and dense PEG brushes could release more trapped water molecules to resist BSA adsorption. Preadsorbed Fib could be gradually displaced by high-concentration BSA, while the adsorption and displacement of Fib was determined by surface hydrophilicity.

论文链接：https://doi.org/10.1021/la4017239