作者：Jing Jin, Fujian Huang, Yu Hu, Wei Jiang, Xiangling Ji, Haojun Liang, Jinghua Yin
关键字：双偏振干涉, 石英晶体微天平, Pluronic, 蛋白质吸附, 血小板黏附
论文来源：期刊
具体来源：Colloids and Surfaces B: Biointerfaces, 2014, 123, 892-899
发表时间：2014年

摘要：利用双偏振干涉技术（DPI）在线监测了四种Pluronic在疏水表面的固定动力学，并阐明了Pluronic构象对蛋白质吸附的影响。结果表明，疏水链段的比例而非疏水链段长度影响Pluronic的链密度；其固定密度取决于水溶液中PEO水合作用与PPO在基底上疏水相互作用的竞争。P-123因疏水作用占主导而获得最大的接枝质量（2.89±0.25 ng/mm2），其疏水链段在C18基底上缓慢分步锚定；P-123具有最大的疏水链段比例（环氧丙烷/环氧乙烷=3.63），形成刷状链构象，表现出优异的抗蛋白质和抗血小板性能。QCM-D结果进一步证实P-123在基底上呈较伸展的刷状PEO构象，而L-35呈较松散的煎饼状结构；P-123中的PEO通过调节构象维持天然结构并抵抗牛血清白蛋白吸附。因此，固定Pluronic的血液相容性受疏水链段比例及其PEO构象共同影响。

Abstract：Dual polarization interferometry was used to monitor the immobilization dynamics of four Pluronics on hydrophobic surfaces and to elucidate the effect of Pluronic conformation on protein adsorption. The proportion of hydrophobic chain segments, not the length of the hydrophobic chain, influenced the chain densities of the Pluronics. The immobilized densities resulted from competition between the hydration of PEO in aqueous solution and the hydrophobic interaction of PPO on the substrate. P-123 obtained the largest graft mass (2.89±0.25 ng/mm2) because of the dominant effect of hydrophobic interactions, and exhibited the largest hydrophobic chain segment proportion (propylene oxide/ethylene oxide = 3.63), forming a brush chain conformation with excellent protein and platelet resistance. QCM-D further confirmed that the PEO conformation in P-123 on the substrate was a relatively extended brush chain, whereas L-35 showed relatively loose pancake-like structures. The PEO in P-123 regulated the conformation to maintain the native conformation and resist the adsorption of bovine serum albumin. Thus, the hemocompatibilities of the immobilized Pluronics were influenced by the proportion of hydrophobic chain segments and their PEO conformations.

论文链接：https://doi.org/10.1016/j.colsurfb.2014.10.038