作者：Yu Hu, Jing Jin, Yuanyuan Han, Jinghua Yin, Wei Jiang, Haojun Liang
关键字：纤维蛋白原, 蛋白质吸附, 聚乙二醇, 石英晶体微天平, 双偏振干涉, 接枝密度
论文来源：期刊
具体来源：RSC Advances, 2014, 4(15), 7716-7724
发表时间：2014年

摘要：蛋白质吸附行为是决定材料能否用作医用高分子材料的关键因素。本文利用带耗散功能的石英晶体微天平（QCM-D）和双偏振干涉技术（DPI），从吸附质量、黏弹性及链构象等方面研究了纤维蛋白原（Fib）在三种链长和链密度不同的聚乙二醇（PEG）表面上的吸附。在QCM-D芯片上，PEG链呈紧密伸展的刷状构象；在DPI芯片上，PEG1000和PEG2000可能具有相似的煎饼状构象，而PEG5000呈蘑菇状构象。在较疏松的煎饼状PEG1000和PEG2000改性DPI表面上存在裸露区域；Fib可在相对致密的PEG1000表面充分铺展，并在相对稀疏的PEG2000表面部分铺展、紧密取向。因此，当亲水分子链构象为疏松煎饼状时，接枝密度通过影响Fib的铺展对吸附抵抗性起更重要作用。此外，刷状和蘑菇状的PEG5000链均具有较高变形能力，可通过调整构象减少与Fib的相互作用而优异抵抗蛋白质吸附。PEG改性表面对Fib的吸附抵抗性取决于PEG层的接枝密度和PEG链的变形能力。

Abstract：Fibrinogen (Fib) adsorptions on three different poly(ethylene glycol) (PEG) surfaces that differed in chain length and chain density were investigated using a quartz crystal microbalance with dissipation (QCM-D) and a dual polarization interferometry (DPI) with respect to adsorbed masses, viscoelastic properties and chain conformations. On QCM-D chips, PEG chains were tight and in extended brush conformations, while on DPI chips, PEG1000 and PEG2000 may have the same pancake-like conformations but PEG5000 had a mushroom conformation. Several bare spaces were observed on the loose pancake-like PEG1000- and PEG2000-modified DPI surfaces. Fib could fully spread on the relatively dense PEG1000-modified DPI surface and partly spread and tightly orient on the relatively sparse PEG2000-modified DPI surface. Grafting density was found to have greater significance in determining Fib adsorption resistance due to its influence on Fib spreading when the chain conformations of hydrophilic molecules were loose pancake-like structures. Furthermore, brush and mushroom structured PEG5000 chains both had high deformation capacity, which excellently resisted protein adsorption by adjusting their conformation to decrease interaction with Fib. Therefore, the Fib adsorption resistance of PEG-modified surfaces depended on the grafting density of the PEG layer and the deformation capacity of the PEG chain.

论文链接：https://doi.org/10.1039/c3ra46934d