作者：Jing Jin, Jiao Ma, Lingjie Song, Wei Jiang, Thomas Ederth
关键字：无纺布聚丙烯, 光接枝, QCM-D, 免疫效率, 免疫分析平台
论文来源：期刊
具体来源：Materials Science and Engineering: C, 2019, 102, 492-501
发表时间：2019年

摘要：免疫分析检测平台技术对临床疾病诊断至关重要，尤其是对开发医疗诊断系统。本文利用光接枝反应在无纺布聚丙烯（PP）上接枝甲基丙烯酸2-羟乙酯（HEMA）和磺基甜菜碱（SBMA），制备了基于聚合物的免疫分析平台。通过纤维蛋白原吸附和血小板黏附考察了PP-g-P(HEMA-co-SBMA)的防污性能，并用羰基二咪唑活化HEMA链段上的悬挂羟基以共价偶联抗体分子。免疫分析平台的检测限低至10 pg/mL。抗体数量和生物活性影响抗体的可用性和免疫分析灵敏度，免疫效率取决于抗体固定化策略。QCM-D测得Au-g-P(SBMA-co-HEMA)和Au-SH表面的免疫效率分别为165%和35.7%。通过亲水性聚合物链作为间隔层共价结合抗体，可保持片段抗原结合区向上取向、维持抗体生物活性，并主要通过调节聚合物链构象提高抗体分子在识别抗原时的可及性。因此，将两性离子PSBMA和反应性PHEMA等亲水聚合物接枝到无纺布PP上并以共价策略结合抗体，有望开发成商用免疫分析平台。

Abstract：The technology of an immunoassay detection platform is critical to clinical disease diagnoses, especially for developing a medical diagnostic system. A polymer-based immunoassay platform was fabricated on nonwoven fabric polypropylene (PP) using a photografting reaction to graft 2-hydroxyethyl methacrylate (HEMA) and sulfobetaine (SBMA). The antifouling properties of PP-g-P(HEMA-co-SBMA) were investigated by fibrinogen adsorption and platelet adhesion. Carbonyldiimidazole was employed to activate the pendant hydroxyl groups in HEMA moieties and covalently coupled antibody molecules. The detection limit of the immunoassay platform was as low as 10 pg/mL. Antibody amount and bioactivity affected the availability of antibody and the sensitivity of immunoassay. The immune efficiency was dependent on the strategies of antibody immobilization. The immune efficiency of Au-g-P(SBMA-co-HEMA) and Au-SH surfaces measured by QCM-D was 165% and 35.7%, respectively. The covalently binding antibody via hydrophilic polymer chains as spacers could retain fragment antigen-binding up orientation, maintain the bioactivity of antibody, and mainly improve the accessibility of antibody molecules via adjusting the conformations of polymer chains when the antibodies recognized the antigens. Therefore, grafting hydrophilic polymers, such as zwitterionic PSBMA and reactive PHEMA onto nonwoven fabric PP, and binding antibody by covalent strategy had the potential to be developed as a commercial immunoassay platform.

论文链接：https://doi.org/10.1016/j.msec.2019.04.082