作者：Jianing Zhang, Jiayu Yu, Jing Jin, Xiang Zhou, Haojun Liang, Feng Zhou, Wei Jiang
关键字：桥连DNA, 纳米粒子组装, 化学反应网络, 链置换反应, 分子机器
论文来源：期刊
具体来源：Nanoscale, 2022, 14(33), 12162-12173
发表时间：2022年

摘要：桥连DNA是编程化学反应网络的重要结构。本文构建了桥连DNA引导的纳米粒子组装，通过toehold介导链置换反应编程一步和多步反应，以获得更高的结构复杂性和动态调控行为。桥连DNA连接体的结构和toehold长度对成功构建分子机器和实现多步反应具有重要影响。六个碱基的toehold即可在桥连DNA中实现toehold介导链置换反应；当toehold长度2与toehold长度1之差大于等于1时，多步反应可由桥连DNA驱动触发并进行。应用方面，通过改变桥连DNA上的四个单链尾，可同时检测两条靶DNA并构建逻辑门。原则上，这种桥连DNA引导AuNPs组装的方法可实现任何可用数学表达的行为。

Abstract：Bridge DNA is an essential structure for programming chemical reaction networks. In this work, a bridge DNA guided assembly of nanoparticles has been constructed to program one-step and multi-step reactions via toehold-mediated strand displacement reaction for higher structural complexity and dynamic regulation behaviors. The structures of the bridge DNA linker and the length of the toeholds have an essential effect on successful construction of a molecular machine and achievement of multi-step reactions. A six-base toehold is enough to achieve the toehold-mediated strand displacement reaction in bridge DNA. When the difference between toehold length-2 and toehold length-1 is equal to or larger than one, the multi-step reaction can be triggered and performed by the driving of bridge DNA. For application, both simultaneous detection of two target DNA strands as well as the construction of logic gates can be achieved by changing the four single-stranded tails on the bridge DNA. In principle, this approach of the bridge DNA guiding the assembly of AuNPs can implement any behavior that can be expressed mathematically.

论文链接：https://doi.org/10.1039/d2nr03948f