writer：Mengyi Li, Xiaoyan Zhou, Xiaolong Zeng, Changyong Wang, Jiake Xu,Dong Ma, Wei Xue
keywords：targeting, hyperbranched poly(amido amine)s, cancer therapy
source：期刊
Issue time：2016年

For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized

by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N0-cystaminebisacrylamide.

Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched

poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection

efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in

the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis

of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than

PEI-25k, suggesting a potential application in gene therapy for tumors.