writer：2. Changguo Xue, Tiegui Nan, Shangquan Wu, Zhen Cao, Qingchuan Zhang, Baomin Wang
keywords：Microcantilever biosensor, Ethyl parathion, Antibody, Self-assembled monolayer
source：期刊
specific source：Asian Journal of Chemistry
Issue time：2012年

A microcantilever biosensor has been developed for label-free detection of ethyl parathion (EP). Through self-assembled monolayer(SAM) method, anti EP antibody was immobilized on the gold surface of the microcantilever for the measurement of EP. The deflection of the microcantilever corresponding to different EP concentration was real time monitored by optical lever technique. The experimental results of non-competitive enzyme-linked immunosorbent assay(ELISA) indicate that anti EP antibody on the gold surface can keep the activity. Deflection of microcantilever is linear to EP concentration from 500-2000 ng/mL. The limit of detection is 500 ng/mL.