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Thermally responsive silicon nanowire arrays for native/denatured-protein separation
作者:Hongwei Wang, Yanwei Wang, Lin Yuan, Lei Wang, Weikang Yang, Zhaoqiang Wu, Dan Li and Hong Chen
关键字:温敏性聚合物,PNIPAAm, 硅纳米线阵列,蛋白质吸附,温度循环
论文来源:期刊
具体来源:Nanotechnology
发表时间:2013年
We present our findings of the selective adsorption of native and denatured proteins onto thermally responsive, native-protein resistant poly(N-isopropylacrylamide) (PNIPAAm) decorated silicon nanowire arrays (SiNWAs). The PNIPAAm–SiNWAs surface, which shows very low levels of native-protein adsorption, favors the adsorption of denatured proteins. The amount of denatured-protein adsorption is higher at temperatures above the lower critical solution temperature (LCST) of PNIPAAm. Temperature cycling surrounding the LCST, which ensures against thermal denaturation of native proteins, further increases the amount of denatured-protein adsorption. Moreover, the PNIPAAm–SiNWAs surface is able to selectively adsorb denatured protein even from mixtures of different protein species; meanwhile, the amount of native proteins in solution is kept nearly at its original level. It is believed that these results will not only enrich current understanding of protein interactions with PNIPAAm-modified SiNWAs surfaces, but may also stimulate applications of PNIPAAm–SiNWAs surfaces for native/denatured protein separation.