作者：Xue-meng Fan, Hou-Yong Yu, Duan-Chao Wang, Juming Yao, Haitao Lin, Chunxia Tang, and Kam (Michael) C
关键字：Cellulose nanocrystals, fluorescein isothiocyanate, photoluminescence quantum yield, cellular uptake, dispersion evaluation
论文来源：期刊
具体来源：ACS Applied Materials & Interfaced
发表时间：2019年

Spherical cellulose nanocrystals (SCNs) and rod-shaped cellulose nanocrystals (RCNs) were

extracted from different cellulose materials. The two shape forms of cellulose

nanocrystals (CNs) were designed with combination of isothiocyanate (FITC),

both the obtained FITC-SCNs and FITCRCNs exhibited high fluorescence

brightness. The surfaces of SCNs and RCNs were subjected to a secondary imino

group by a Schiff reaction, and then covalently bonded to the isothiocyanate

group of FITC through a secondary imino group to obtain a fluorescent cellulose

nanocrystal (FITC-CNs).The absolute zeta potential and dispersion stability of

FITC-CNs (FITC-SCNs and FITC-RCNs) were improved, which also promoted the

increase in fluorescence quantum yield. FITC-RCNs had a fluorescence quantum

yield of 30.7%, and FITC-SCNs had a morphological advantage (better dispersion,

etc.), resulting in higher fluorescence quantum yield of 35.9%. Cell

cytotoxicity experiments demonstrated that the process of FITC-CNs entering

mouse osteoblasts (MC3T3) did not destroy the cell membrane so showed good

biocompatibility. On the other hand, FITC-CNs with good dispersibility can

significantly enhance polyvinyl alcohol (PVA) and polylactic acid (PLA), their

mechanical properties were improved (the highest sample reached to 243%), and

their fluorescent properties were imparted. This study provides a simple

surface functionalization method to produce high-luminance fluorescent

materials for bioimaging, multifunctional nano enhancement/dispersion marking

and anti-counterfeiting materials.