作者：Yu Xiao, Tao Gong, Shaobing Zhou*
关键字：Nanotubes,Biomineralization
论文来源：期刊
具体来源：Biomaterials 31 (2010) 5182e5190
发表时间：2010年

A simple and effective approach was introduced to functionalize multi-walled carbon nanotubes
(MWNTs) by in situ deposition of hydroxyapatite (HA) to improve their hydrophilicity and biocompatibility.
Firstly, we prepared two types of pre-functionalized MWNTs: acid-oxidated MWNTs and covalently
modified MWNTs by poly (ethylene glycol) (PEG). The influences of the acid-oxidated time, prephosphorylation,
and PEGylation of MWNTs on in situ growth of HA were further investigated in
simulated body fluid (SBF) with ionic concentration: 2, 5 and 10 times, respectively, at 37
C for 24 h. The
results exhibited that all these factors have positive effects on the HA crystals growth, especially the
PEGylation of MWNTs plays a key role during the deposition. Finally, the methyl thiazolyl tetrazolium
(MTT) assay was performed to evaluate their cytotoxicity, which showed that the PEGylated MWNTs
wrapped by HA crystals have the best biocompatibility.