writer：Wu, Wei-Bing1; Wang, Ming-Liang ; Sun, Yue-Ming; Huang, Wei; Cui, Yi-Ping; Xu, Chun-Xiang
keywords：Two-stage dispersion polymerization; Fluorescent microspheres; Tunedcolor; Fluorescence resonance energy transfer; 1,8-Naphthalimide
source：期刊
specific source：Optical Materials, Volume 30, issue 12 (August, 2008), p. 1803-1809.
Issue time：2008年

Optical Materials, Volume 30, issue 12 (August, 2008), p. 1803-1809.

http://www.sciencedirect.com/science/article/pii/S0925346707003357

Fluorescent monodisperse polystyrenemicrospheres were prepared by two-stage dispersion polymerization, which successfully covalently labeled microspheres with two dyes without disturbing the final particle size and size distribution. By varying the dye concentrations, microspheres show tunedcolors with different fluorescent intensity under a singlewavelengthexcitation. Fluorescence resonance energy transfer (FRET) between two labeled dyes was proved to contribute to the emission of the longer-wavelength dye at a shorter-wavelengthexcitation. There is no dye leakage for microspheres because of the covalent incorporation of dye molecules. The microsphere matrix provides good protection of dye molecules and blocks the influence of media outside on the fluorescence of microspheres. Single microsphere shows intense fluorescence due to a large number of encapsulated dye molecules. These uniform barcoding fluorescent microspheres have potential application in multiplexed bioanalysis.