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Photoinduced inhibition of DNA unwinding in vitro with water-soluble polymers containing both phosphorylcholine and photoreactive groups
作者:Xiaojie Lin, Kyoko Fukazawa, Kazuhiko Ishihara
关键字:Methacryloyloxyethyl phosphorylcholine polymer, Photoreactivity, Cell membrane permeation, Molecular beacons, Regulation of DNA unwinding
论文来源:期刊
具体来源:Acta Biomaterialia 40 (2016) 226–234
发表时间:2016年

Nile blue (NB)-tagged DNA helix-targeting amphiphilic photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, poly(MPC-co-3-methacryloyloxy-2-hydroxypropyl-4-oxybenzophenone-co-2-trimethylammonium ethyl methacrylate chloride) (PMHT–NB), containing a cationic group to facilitate cell membrane penetration and a benzophenone (BP) group to promote photoinduced conjugation with DNA helix was synthesized using radical polymerization method. Ultraviolet light (UV)-visible light absorption spectra of PMHT–NB showed absorption peaks at wavelengths 254, 289, and 600 nm, suggesting successful incorporation of BP and NB groups. PMHT–NB was highly sensitive to photoirradiation with UV irradiation at the second level, as confirmed based on the degradation spectra of UV absorption peaks for the BP group in PBS (pH = 7.4). PMHT–NB showed good solubility in both aqueous solution and in ethanol. In a cell culture medium containing 10 mg/mL PMHT–NB, the NB group showed fluorescence peaks at an emission wavelength of 650 nm and excitation wavelength of 633 nm. PMHT–NB also showed low cytotoxicity and good cell membrane permeability toward cancerous HeLa cells. Further, photoinduced PMHT–NB effectively inhibited the unwinding of a molecular beacon with a hairpin structure, indicating that synthetic photoreactive MPC polymers photoregulated the unwinding of DNA.