作者：Ruirui Liu, Jian Zhao, Qian Han, Xinyi Hu, Dong Wang, Xu Zhang, Peng Yang*
关键字：particle surface engineering; lysozyme phase transition; amyloid; surface immobilization of living cells; surface modification of living cells
论文来源：期刊
具体来源：Advanced Materials
发表时间：2018年

Advances in material design and applications are highly dependent on the development of particle surface engineering strategies. However, few universal methods can functionalize particles of different compositions, sizes, shapes, and structures. We describe the amyloid-like protein assembly-mediated surface modification and functionalization of particles and living colloids with different compositions, sizes, shapes, and structures. The simple one-step incubation of inorganic, polymeric or metal micro/nanoparticles in a unique amyloid-like phase-transition buffer containing lysozyme led to the rapid formation of a robust proteinaceous coating on the particle surfaces. This nanoscale phase-transitioned lysozyme (PTL) coating presented strong binding with particle surfaces to resist mechanical and chemical peeling under harsh conditions and versatile surface functional groups to support a series of sequential surface chemical derivatizations, such as radical living graft polymerization, the electroless deposition of metals, biomineralization and the facile synthesis of Janus particles and metal/protein capsules. Being distinct from other methods, the preparation of this pure protein coating under biocompatible conditions (e.g., neutral pH and nontoxic reagents) provides a reliable opportunity to directly modify living cell surfaces without affecting their biological activity. The PTL coating armed yeasts with a functional shell to protect their adhered body against foreign enzymatic digestion. The PTL coating further supported the surface immobilization of living yeasts for heterogeneous microbial reactions and the sequential surface chemical derivatization of the cell surfaces, e.g., radical living graft polymerization. The establishment of this new surface engineering technique for particles and living cells can lead to a diverse array of tools for chemists, which may drive a variety of applications ranging from biomedicine to life science, energy, environmental and catalysis applications.