A highly selective fluorescent nanoprobe based on AIE and ESIPT for imaging hydrogen sulfide in live cells and zebrafish
作者：Peisheng Zhang, Xuezheng Nie, Meng Gao, Fang Zeng,* Anjun Qin, Shuizhu Wu* and Ben Zhong Tang
关键字：hydrogen sulfide, AIE, nanoprobe
论文来源：期刊
具体来源：Mater. Chem. Front., 2017,1, 838-845
发表时间：2017年
The rational design of effective tools capable of monitoring hydrogen sulfide (H2S) is of great
importance to fully understand its physiological and pathological functions. Herein, a self-assembled fluorescent nanoprobe with both aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics for H2S detection has been successfully developed via a modified nanoprecipitation method. The nanoprobe features high water dispersibility, a large Stokes shift (ca. 100 nm), good biocompatibility as well as high selectivity towards H2S over other putative interferents. Live cell imaging experiments demonstrate that the nanoprobe, with good cell-membrane permeability, can facilitate the visualization of exogenous and endogenous H2S levels. Moreover, the nanoprobe has also been found capable of detecting H2S in zebrafish. This nanoprobe with AIE and ESIPT characteristics can provide a novel method for the development of fluorescent probes for monitoring biological processes.