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Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots
作者:Hong Wang, Peisheng Zhang,* Yongxiang Hong, Bin Zhao, Pinggui Yi and Jian Chen*
关键字:HClO, polymer dots, Lysosome,
论文来源:期刊
具体来源:Polym. Chem.,2017, 8,5795–5802
发表时间:2017年

The development of an effective method for imaging of lysosomal hypochlorous acid (HClO) is of great significance to better understand the roles of HClO-related diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET)-based fluorescent polymer dots (FPD) for the ratiometric imaging of lysosomal HClO in living cells via a simple co-precipitation method. This FRET-based construction strategy takes the advantage of flexible design by incorporating target-inert fluorogen (donor) and target-sensitive fluorogen (acceptor) into the nanoparticle to avoid leakage and photobleaching problems. The as-prepared FPD exhibits good water dispersibility, fast response (<30 s), high sensitivity (~5.1 nM), outstanding selectivity, excellent photostability and long-term stability (>15 weeks). Moreover, the resultant FPD with good membrane permeability could mainly locate in the lysosomes and act as a probe for the ratiometric imaging of lysosomal HClO in living HeLa cells.