您所在的位置：首页 > 科学研究 > 学术论文 > 高分子工程 > Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy
Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy
Fabricating a cycloolefin polymer immunoassay platform with a dual-function polymer brush via a surface-initiated photoiniferter-mediated polymerization strategy
The development of technologies for abiomedical detection platform is critical to meet the globalchallenges of various disease diagnoses. In this study, an inertcycloolefin polymer (COP) support was modified with two-layerpolymer brushes possessing dual functions, i.e., a lowfouling poly[poly(ethylene glycol) methacrylate] [p-(PEGMA)] bottom layer and a poly(acrylic acid) (PAA)upper layer for antibody loading, via a surface-initiatedphotoiniferter-mediated polymerization strategy for fluorescence-based immunoassay. It was demonstrated through aconfocal laser scanner that, for the as-prepared COP-g-PEG-b-PAA-IgG supports, nonspecific protein adsorption was suppressed,and the resistance to nonspecific protein interference on antigen recognition was significantly improved, relative to the COP-g-PAA-IgG references. This strategy for surface modification of a polymeric platform is also applicable to the fabrication of otherbiosensors.
