Bacterial cellulose (BC) has been proposed as a biomaterial applied in biomedical scope due to its goodbiocompatibility. Recent reports showed that human adipose-derived stem cells (HASCs) have become anew choice to be used as seeding cells in tissue engineering. The objective of this study is to explore thepotential of using BC and HASCs as scaffold and seeding cells in bone tissue engineering. The osteogenicdifferentiation was investigated by Von Kossa, Alizarin Red, ALP cellular staining and RT-PCR. The resultsshowed that HASCs took a successful osteogenic differentiation on BC. Moreover, the in vivo animal test
also provided the confirmation of the repair ability of BC on damaged bone. In conclusion, the authordemonstrates the osteogenic differentiation of HASCs on BC and the feasibility of using BC and HASCs asscaffold and seeding cells in bone tissue engineering.
